Comparison of Actinobacterial Diversity in Jiangcheng and Heijing Saline Mines in Yunnan by Using Culture-independent Approach

Abstract:

Actinobacterial diversity in samples of saline mines was investigated by constructing three actinobacterium-specific 16S rRNA gene clone libraries from Jiangcheng salt mine and Heijing brine pits. The primers for the Class Actinobacteria were used to construct the 16S rRNA gene clone libraries. At least 469 purified clones were digested with Hae III for RFLP analysis, in order to separate the clones into groups according to their restriction patterns. Among of them, 133 sequences were selected and determined for their partial 16S rRNA gene sequences. The result of phylogenetic and statistic analyses indicated that actinobac- terial OTUs were closely related to most members of the Class Actinobacteria, and some OTUs may represent some unknown groups. Heijing and Jiangcheng located in different saline places of Yunnan, China. However, the communities, species diversity of Actinobacteria and Phylogenetic relationship from the two saline mines were similar.