Four kinds of colophony were used to isolate the herbicidal substance from the toxin produced by isolate PAM1 of Pythium aphanidermatum, which had herbicidal activity to Digtaria sanguinealis L and the results showed X-5 was the best one for absorbing herbicidal substance among the four kinds of colophony used. Four solvents including 50% ethanol, 95% ethanol, ethyl acetate and acetone were used as eluting solvent and 2 procedures were tested, and the results of growth inhibition and seed germination indicated that the best eluting procedure was procedure 1.
ZHANG Li-Hui, WANG Yi-Jing, ZHANG Jin-Lin, DONG Jin-Gao. Colophony Adsorbing Isolating Herbicidal Substance from Mycotoxin Produced by Pythium aphanidermatum[J]. Microbiology China, 2008, 35(9): 1505-1509
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