Use of Fluoresceinated Recombinant Phage to Study the Receptor/Ligand Interaction

Abstract:

VP2 protein of infectious bursal disease virus(IBDV) was displayed on T7 phage surface, and this recombinant phage was then purified and labeled with FITC. The interaction between fluorescigenic phage and IBDV host cells was detected by fluorescent microscope and flow cytometer(FCM). Data shows that after displayed on labeled T7 phage suface, VP2 protein still remains its binding activities with IBDV host cells, and this interaction can be blocked by IBDV vaccine strain TAD in a does dependent manner, while no interaction was observed in negative control. The described method should find widespread application in the rapid in vivo research of interactions between ligands and receptors.