The New Testing Methods of Nematode-trapping Fungi to Pine Wood Nematode Bursaphelenchus xylophilus

Abstract:

According to the predacity of the nematode-trapping fungi and the growth habit of pine wood nematode Bursaphelenchus xylophilus, this paper tested the B. xylophilus that cultured by the fixed time and quantities. Trying to find out the quantificational test method of trapping rate, by the change of the quantities of B. xylophilus. Three new methods of testing trapping rate were obtained successfully basing on the improvement of Baermann funnel method. Each method had advantage and disadvantage, Filter membrane and Double layer microscopic examination method were more precise, and bar-cutting microscopic examination and Baermann funnel method were more imprecise. Therefore, according to the emphasis point of experiment and precision degree of the test, the more suitable method for the test of trapping rate could be chosen.

Key words:Nematode-trapping fungi, Pine wood nematode Bursaphelenchus xylophilus, Trapping rate, Method