Preliminary Research on the Flora of Endophytic Bacteria and Selection of Useful Endophytic Bacteria in the Seedling of Maize

Abstract:

In the experiment, 68 endophytic bacteria strains of maize were isolated and purified from seedlings of three maize cultivars (Chuandan13, Chuandan418 and Chuandan416). Subsequently, these bacteria were identified and studied. The results indicated that these bacteria belong to five different genera. Among them, Bacillus, Micrococcus was the most widely distributed and predominant. Bacilli were classified into 5 species level. The endophytic bacteria isolated from three maize cultivars were variable, and the number of different species population of these endophytic bacteria followed as chuandan13>chuandan418>chuan- dan416. According to the inhibitory spectrum and influence of endophytic bacteria fermentation to the corn seeds, two strains (BH and B98) had wide pathogen range and didn’t inhibit the viability of the seeds under intraventricular condition; the pot experiment results indicated both of the two strains could promote maize growth and antagonize the Rhizoctonia solani.

Key words:Corn sheath blight, Rhizoctonis solani, Endophytic bacteria, Promoting and inhibiting