Synthesis of Medium-chain-length Polyhydroxyalkanoate (PHAMCL) by Pseudomonas mendocina NK-01

Abstract:

A strain which can synthesize medium-chain-length polyhydroxyalkanoate (PHAMCL) was isolated from soil. It was named as Pseudomonas mendocina NK-01 through classification and identification. The PHAMCL was synthesized by P. mendocina NK-01 from glucose in the condition of limited nitrogen source. The structure of it was characterized. In order to construct a recombined plasmid pBSphaC1, an expressing plasmid of E.coli, after phaC1 gene encoding PHA synthase was cloned from Pseudomonas mendocina NK-01 by PCR amplification, it was linked into pBluescript SK—. PhaC1 gene was expressed successfully in E.coli JM109. Fermentation synthesis was also investigated by recombined E.coli.