The Cultural Characteristics and Stability of the Dualfunctional Bacteria Community NSC-7

Abstract:

The cultural characteristics and stability of the bacteria community NSC-7, which was capable of degrading cellulose and lindane with high efficiency was discussed in this study. NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatography mass spectrometry (GC-MS), and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80℃ and freeze-drying for four years. It could remain its degrading ability after 90℃ of high temperature treating for 30 min, and resume its degrading ability after subcultured 2 times after 105℃ of high temperature treating for 30 min in autoclave. It suggested that NSC-7 had efficient conservation stability and thermo-stability. DGGE analysis showed that the composition of NSC-7 remained stable after subcultured for several times.

Key words:Cellulose degradation, Lindane degradation, Bacteria community NSC-7, Stability