Transformation and Identification of SCAR Marker from Specific RAPD Marker of Bacillus TS02

Abstract:

Bacillus cereus TS02 is a functional strain separated by our laboratory. RAPD was used to analyse the specificity of TS02 in contrast to other five Bacillus bacteria (Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus megaterium and Bacillus pumilus) that are highly homologic to TS02 according to the conventional taxonomy. A specific marker TSR1 (533 bp) was obtained, cloned and sequenced, a specific primer pair P1/P2 was designed based on the TSR1 sequence. P1/P2 can specifically amplify the objective band which is only in TS02 and not found in other control strains. The results showed that the specific RAPD marker had been transformed to the steady SCAR marker, and the specific SCAR marker can correctly detect the Bacillus TS02 in specie’s lever.