Method of Extract Genomic DNA of Yeast for AFLP

Abstract:

The high molecular weight genomic DNA of yeast was extracted using three methods. Products were separated on agarose gel electrophoresis, quantified by spectrophotometer ND-1000 and restricted by EcoRⅠand MesⅠ. The result was shown that the genomic DNA extracted by modified benzyl chloride method was the best. The products of wild isolates supported it, too. This method was suitable for restriction of genomic DNA from yeast.