Method of Extract Genomic DNA of Yeast for AFLP

Home > Archive>Volume 35, Issue 4, 2008 >0629-0632

Method of Extract Genomic DNA of Yeast for AFLP
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                        LIU Yun-PengLIU Yun-Peng
Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018
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NI Hui-JuanNI Hui-Juan
Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018
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SUN Tian-SongSUN Tian-Song
Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018
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YU JieYU Jie
Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018
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ZHANG He-PingZHANG He-Ping
Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018
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Abstract:

The high molecular weight genomic DNA of yeast was extracted using three methods. Products were separated on agarose gel electrophoresis, quantified by spectrophotometer ND-1000 and restricted by EcoRⅠand MesⅠ. The result was shown that the genomic DNA extracted by modified benzyl chloride method was the best. The products of wild isolates supported it, too. This method was suitable for restriction of genomic DNA from yeast.

Key words:Yeast, DNA extract, Benzyl chloride, AFLP analysis

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LIU Yun-Peng, NI Hui-Juan, SUN Tian-Song, YU Jie, ZHANG He-Ping. Method of Extract Genomic DNA of Yeast for AFLP[J]. Microbiology China, 2008, 35(4): 0629-0632

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