Analysis of Signal Peptide Fragment of Canine Distemper Virus Wild-type Strain Fusion Protein

Abstract:

A pairs of primers were designed according to the fusion protein (F) gene sequences of canine distemper virus (CDV) in GenBank. A 369 bp fragment aimed signal peptide fragment of F gene was amplified. The PCR products from viscera samples, blood, urine of fur animals including foxes, minks and raccoon dogs, which collected in the years 2005-2007, were cloned to pMD18-T Vector and sequenced. We obtained 13 positive signal peptide fragments from wild-type strains. The results indicated there was obviously genetic diversity between the wild-type strains and CDV3 and other vaccine strains. The homology with CDV3 is 80.7%－83.2% in nucleotide, and 64.8%－71.3% in amino acid. The analysis for the hydrophobic regions indicated the function of signal peptide fragment may be changed. This study can offer academic data to research of CDV genetic variation and epidemiology.

Key words:Canine distemper virus, Fusion protein, Signal peptide