Isolation of Plasma Membrane of Antarctic Yeast by Two-phase Partition

Abstract:

Plasma Membrane of Antarctic yeast Rhodotorula sp. NJ298 was purified from microsome in a two-phase aqueous system including Dextran T 500 and PEG 3350. The effects of the polymer and KCl concentration in the system on the plasma membrane partition were studied. The result indicated that the system with 6.0% (W/W) Dextran T 500 and PEG 3350, 4 mmol/L KCl was suitable for the plasma membrane isolation in Rhodotorula sp. NJ298. The electron micrograph stained with phosphotungstic acid and the purity identification analyzed by marker enzyme activities proved that the above two-phase system could obtain sealed positive plasma membrane vesicles with the purity of up to 78.2%. This paper was a basic about studying adaptation mechanism of Rhodotorula sp. NJ298 by isolation the plasma membrane.