The Advancement about Baculovirus Surface Display System

Abstract:

At present, microbe surface display system mainly involves phage surface display system, bacterial surface display system, yeast surface display system and virus surface display system. Baculovirus surface display system is a new type of eukaryote surface display system which developed based on deeply understanding of construction and function of virus genome in recent years. Through fused expression with viral capsid or membrane proteins exogenous peptides can be displayed on the surface of the virus and formed hedgehog-shape “fake virus”. Baculovirus surface display system was characterized by safeness and high performance, furthermore, this system can complete post-translation processing and modification of protein to enhance the bioactivity of exogenous product. Combined with the author’s experimental work, this paper briefly introduces the mechanism and traits of this system and summarizes the newest research development on its application in the field of monoclonal antibody preparation, new-type vaccine development, genes transduction and genes therapy. It is believed that the system above may show extensive application through further improvement and optimization.