Metabolic Flux Analysis of L-Arginine Fermentation in Corynebacterium glutamicum

Abstract:

In this paper, metabolic networks of the Corynebacterium glutamicum GWY020 and the two derivatives carrying additional mutations HUI821and GUI089 were established and modified. The concentrations of extra-cellular metabolites were determined under sub-steady-state (50 h~52 h) of the batch culture. The metabolic flux distribution maps of the three strains were obtained, compared and analyzed. These results indicate that the introduction of analog supersensitive marker or analog resistant marker skew the metabolic flux towards the formation of L-Arginine. This study revealed the usefulness of the metabolic flux analysis as a tool for verification of existing production strains. The analysis may play an important role in helping us to rationally re-design metabolism for further improvement of fermentation process.

Key words:L-Arginine, Corynebacterium glutamicum, Metabolic flux analysis