Typing of Vibrio parahaemolyticus by Repetitive Element Sequence-based PCR

Abstract:

Two kinds of Rep-PCR, Repetitive extragenic palindromic elements-PCR(REP-PCR) and enterobacterial repetitive intergenic consensus sequences-PCR (ERIC-PCR), were used firstly in typing and genetic relationship research for Vibrio parahaemolyticus in China, and Hunter＆Gaston’s method was used to calculate discriminative index of REP-PCR and ERIC-PCR. Forty Vibrio parahaemolyticus strains, representing a wide range of REP and ERIC patterns, were grouped into 21 and 4 patterns with discriminative index of 0.953 and 0.5, by REP-PCR and ERIC-PCR respectively. G1 type was predominant in 40 strains of Vibrio parahaemolyticus when typed by REP-PCR. The results show that Rep-PCR is feasible for typing Vibrio parahaemolyticus strains, and REP-PCR possesses better typing potential. Similar DNA fingerprints were gained from serotype O1 and O3 strains by two kinds of Rep-PCR. This result indicate that the genetype of serovar O3 and O1 are closely related.

Key words:Vibrio Parahaemolyticus, Repetitive extragenic palindromic elements-PCR, Enterobacterial repetitive intergenic consensus sequences-PCR, Typing, Genetic relationship