Integration and Expression of BGL1 Gene from Saccharomycopsis fibuligera in Industrial Saccharomyces cerevisiae

Abstract:

The recombinant plasmid pPIC-gpd-bgl-hyg was constructed, which contained GPD2 promotor and terminator from industrial yeasts Saccharomyces cerevisiae, b-glucosidase gene (BGL1) from Saccharomycopsis fibuligera and hyg from hygromycin as the selected marker. With the yeast’s high efficiency of homologous integrated, the BGL1 gene was successfully integrated into industrial yeasts S. cerevisiae. The recombined yeast could grow on the cultures with the cellobiose as a sole carbon source, and the β-glucosidase activity achieved 0.764 U/mL after 48 hours’ cultivation. In the experiments of VHG ethanol fermentation, the cellobiose concentration in broth of recombined yeast was 80% lower than that of industrial yeast.

Key words:Industrial yeast, β-glucosidase, Homologous integrated, Hygromycin B