Isolation and 16S rRNA Gene Sequence Analysis of a Thermotolerant Halophilic Bacterium

Abstract:

A strain of thermotolerant halophilic bacteria YJ0238 was isolated from the salt well at 47℃ in the Kangning countryside where was located at the side of Lancang River in the Tibet Autonomous Region. Some physiological and biochemical properties were characterized.16S rRNA gene sequence of YJ0238 was amplified by PCR, and its nucleotide sequence was determined. Based on it’s physiological and biochemical properties, homology and phylogenetic analysis of 16S rRNA gene sequence, strain YJ0238 was identified as a subspecies of the species Idiomarina zobellii. The GenBank accession number of the 16S rRNA gene sequence of strain YJ0238 is EF693953. Until now, there were few reports on the study of high-temperature and high-salt microbial in domestics. The results of this study will provide research material and information for further studies in this area.