Pyrosequencing technology is famous for its application in detection of SNP site and DNA methylation. At the end of 2005, pyrosequencing technology was used to sequence the whole genome and it has become the most developed next generation high-throughput sequencing technonlogy. In this article, we introduce the theory, operation process and wide application field of the second generation genome sequencer FLX system provided by Roche
XU Guan-Dong. GS FLX, the Leader in Next Generation Sequencer[J]. Microbiology China, 2008, 35(1): 149-151
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