The Optimal Condition of Producing Celluase by Trichoderma aureoviride

Abstract:

Producing cellulase conditions such as different temperature, inoculum size, liquid level and pH level by Trichoderma aureoviride in the shaking bottle were studied by orthogonal design method. The results showed that the main factor affecting the producing cellulase was temperature among the orthogonal conditions. The optimum conditions were as follows: cultivating solution initial pH was 6, cultivating temperature was 28℃, inoculation size was 8%, liquid level was 40 mL in 150 mL triangle bottle, rotation speed was 170 r/min.