Rapid species identification of Mycobacterium tuberculosis by rpoB Gene Micro Array

Abstract:

Rapid species identification of Mycobacterium tuberculosis by rpoB gene micro array. Based on the gene micro array of rpoB, the standard strains of 21 mycobacteria and 8 non-mycobacteria ,126 clinical isolated of mycobacteria were detected by PCR-reverse dot blot hybridization assay. 360bp DNA fragment was amplified from all mycobacteria tested and was not found in all nonmycobacteria except hemolytic streptococcus and corynebacterium pseudodiphtheriticum. The result of specimens were detected by the probe which is composed of 21 oligonucleotide was that probe-Mycobacterium fortuitum cross hybridizated with Mycobacterium platypoecilus while the other probes were specific. The 89 strains of the all 126 strains isolated from clinical specimens were identified to be mycobacterium tuberculosis, the percentage was 70.6%(89/126), while the other 9 strains were identified to be unmycobacterium tuberculosis and the the percentage was 9.2%(9/98). Identification of Mycobacteria by rpoB gene micro array is a rapid and effective method which is of considerable value in clinical territory.