Species diversity of Hygrophorus(Hygrophoraceae, Agaricales) in Shanxi Province and description of Hygrophorus brunneodiscoides sp. nov.

Abstract:

[Background] Hygrophorus, belonging to Basidiomycota, Agaricomycetes, Agaricales, Hygrophoraceae, is widely distributed all around the world. [Objective] To investigate the species of Hygrophorus and enrich the knowledge about the species diversity of this genus in Shanxi Province. [Methods] We collected fungal specimens, observed and characterized their morphology. After DNA extraction, the internal transcribed spacer (ITS) was sequenced. Phylogenetic trees were constructed based on the maximum likelihood method and bayesian inference. [Results] Eleven species of Hygrophorus were identified based on the morphological and molecular evidence, including ten known species and a new species. This article described the macroscopic and microscopic morphological characteristics of the new species Hygrophorus brunneodiscoides in detail. The basidiocarp photos of ten known species and a key to the species of Hygrophorus in Shanxi Province were provided. [Conclusion] We identified a new species, H.brunneodiscoides, in China, confirmed 11 species of Hygrophorus in Shanxi Province, and briefed their geographical distribution and associations with hosts according to the field observation.