Campylobacter jejuni Cia effectors are involved in adhesion and invasion of host cells and activation of inflammatory cytokines
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    Abstract:

    [Background] Campylobacter jejuni, one of the major foodborne pathogens worldwide, can colonize the cecum of poultry, becoming the main source of infection in humans. Currently, the mechanism of C. jejuni colonization in avian intestine has not been elucidated. [Objective] To investigate the roles of different Cia effectors of C. jejuni in the adhesion and invasion of intestinal epithelial cells and immune cells, as well as the activation of inflammatory cytokines, thus elucidating the mechanism of C. jejuni colonization in chicken intestine and providing potential targets for the prevention and control of this pathogen. [Methods] Firstly, homologous recombination was employed to construct gene knockout mutants of different effectors (CiaB, CiaC, CiaD, and CiaI) of the wild-type strain (WT, C. jejuni NCTC 11168). The effects of cia knockout on the biofilm formation and autoagglutination were determined. Subsequently, the adhesion and invasion of cia mutants on intestinal epithelial cell line Caco-2, as well as their invasion in the chicken macrophage line HD11, were determined. Finally, RT-qPCR was employed to determine the expression levels of inflammatory cytokines in HD11 cells infected by the mutants. [Results] The cia gene knockout mutants of C. jejuni were successfully constructed. Compared with WT, ∆ciaD showed reduced biofilm formation, while ∆ciaC, ∆ciaD, and ∆ciaI demonstrated enhanced autoagglutination. The mutant ∆ciaB showcased enhanced adhesion and invasion of Caco-2 cells, while ∆ciaC, ∆ciaD, and ∆ciaI exhibited reduced abilities to adhere and invade Caco-2 cells and invade HD11 cells. The infection of HD11 cells by C. jejuni WT significantly up-regulated the expression levels of different inflammatory cytokines, while ∆ciaB, ∆ciaC, and ∆ciaD showed down-regulated expression levels of different inflammatory cytokines. In contrast, ∆ciaI presented up-regulated expression levels of different inflammatory cytokines. [Conclusion] Different Cia effectors of C. jejuni play different roles in the adhesion and invasion of intestinal epithelial cells and immune cells, as well as the activation of inflammatory cytokine expression. The findings provided a theoretical basis for understanding the interaction mechanism between C. jejuni and host intestinal epithelium, and mining potential targets for the prevention and control of this bacterium.

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WU Jinliang, CUI Yifang, GUO Fangfang, HU Ge, XU Fuzhou. Campylobacter jejuni Cia effectors are involved in adhesion and invasion of host cells and activation of inflammatory cytokines[J]. Microbiology China, 2025, 52(2): 679-689

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History
  • Received:August 30,2024
  • Adopted:October 24,2024
  • Online: February 22,2025
  • Published: February 20,2025
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