Home > Archive>Volume 52, Issue 4, 2025 >1462-1474. DOI:10.13344/j.microbiol.china.240635
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G protein α subunit CgrGa3 regulates vegetative growth, stress responses, conidial production, and pathogenicity of Colletotrichum graminicola
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    Abstract:

    [Background] Colletotrichum graminicola is a pathogenic fungus that poses threats to crops such as Zea mays. Heterotrimeric guanine nucleotide-binding proteins (G proteins) play a crucial role in signal transduction of filamentous fungi, with the G protein α subunit being a major component. [Objective] This study identified the G protein α subunit (group III) CgrGa3 in C. graminicola and investigate its role in the growth and development of this pathogen. [Methods] CgrGa3-deleted mutants were constructed by gene knockout. The phenotypes of the mutants were characterized, including the hyphal growth, stress responses, conidial production, conidial germination, and pathogenicity. [Results] CgrGa3 encoded a protein composed of 355 residues and containing a G_alpha domain. The CgrGa3-deleted mutants exhibited inhibited growth, increased sensitivity to NaCl, KCl and H2O2, and reduced production and germination rates of oval and falcate conidia. Additionally, the pathogenicity of the mutants was markedly weakened. The complementation of this gene restored these phenotypic defects. [Conclusion] CgrGa3 plays a critical role in regulating the vegetative growth, stress responses, asexual development, and pathogenicity of C. graminicola.

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WEI Hanwen, ZHANG Ying, LIN Shaoyuan, ZHOU Shuangzhen, LI Xiaoyu, LIU Zhiqiang. G protein α subunit CgrGa3 regulates vegetative growth, stress responses, conidial production, and pathogenicity of Colletotrichum graminicola[J]. Microbiology China, 2025, 52(4): 1462-1474

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History
  • Received:July 31,2024
  • Adopted:December 13,2024
  • Online: April 21,2025
  • Published: April 20,2025
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