Application of Multilocus Sequence Typing method on Pathogenic Microorganisms Typing and Identification

Abstract:

It is important for identifying, tracking and preventing pathogenic microorganism's outbreak in the epidemiology of infectious diseases. Establish a useful simple method for typing and identifying pathogenic microorganisms is necessary. In 1998, Multilocus sequence typing was proposed which is a nucleotide sequence based approach.It combined developments in high-throughput sequencing techniques with established population genetics.This typing method is a portable and reproducible typing system which can track and investigate pathogenic microbes distributed condition over the global by the internet.Now, MLST schemes have been developed for a variety of prokaryotic and some eukaryotic pathogens (like some fungus).In this paper, the theory of MLST and its application on some pathogenic microbes typing and identifying were introduced.