Analysis of Microbial Community Distribution in Constructed Rapid Infiltrition System(CRI) by DGGE

Abstract:

Sands of different depth were sampled from Constructed Rapid Infiltrition system(CRI) in Shenzhen, and they were analyzed by Denaturing Gradient Gel Electrophoresis(DGGE). The profile of DGGE showed that, the microbial community decreased from top to bottom and in the upper 30cm part, CRI was rich in microbial community about 18 species which played some important roles in the biodegradation of COD, such as Firmicutes、alpha proteobacterium and so on; but less than 12 species existed below 40cm, such as Acidovorax sp.、Nitrospira sp.、Clostridium sp., this indicated that there were some nitrification phenomenon and anaerobic microenvironment in the lower part of CRI. The microbial diversity was prominent different spatially. The results showed the microbial community distribution and were certain meaningful to the stabilization and improvement of CRI.