Studies on Purification and Properties of Phytase from Trichoderma viride

Abstract:

The phytase was extracted from solid state leavening of Trichoderma Viride LH374. The crude product was purified by (NH₄)₂SO₄ precipitation, gel filtration and ion exchange chromatography. The purified phyatse was 13.3 times of the raw products, and the extraction ration was 27.1%. The study on the enzymology of phyatse showed that the optimal temperature and pH were 55℃ and 6.0, respectively. The Km value of the phytase was 0.15mmol/L.