Study on the Rapid Identification of Salmonella by Using Bacteriophage

Abstract:

To study the incidence rate of Salmonella, seven strains bacteria-specific phages as a group were used for identification of Salmonella species. All of the 60 bacteria (for test) were inoculated in Luria Bertani (LB) broth, cultivated in 37℃ for 2 hours, 6 hours, 10 hours and≥18 hours respectively, then tested on agar plate and in LB broth, and made micro-biochemical identification through automatic biochemistry analyzer, to confer the most suitable inoculated hours for test bacteria. The result showed that, tested on agar plate, the positive rates of Salmonella cultivated for 2h, 6h, 10h and ≥18h were 91.1%, 93.3%, 97.8% and 88.9% respectively; while tested in LB broth, a reasonable result was showed in the 10h-inoculated tested bacteria. It was resemble to test with food samples bought from some markets in Nanjing. It provided theoretical evidence for performing detection Salmonella species in food with real time, speed, selective and specific.