Microbial Hydroxylation of Androst-4-ene-3,17-dione I：Screening of Strains and Study of the Transformation Conditions

Abstract:

Microbial hydroxylation of androst-4-ene-3,17-dione(4AD) was investigated in this study.The hydroxylated product of 4AD,11α-hydroxyandrost-4-ene-3,17-dione (11α-OH-4AD) is valuable intermediate in the synthesis of pharmaceuticals. 30 different species and strains of fungi were screened for their biotransformation ability. It was found that Beauveria bassiana QY_{2A strain significantly showed the high 11α-hydroxyalate efficiency and transformate 4AD to the target product 11α-OH-4AD which had been purified and identified.The hydroxylation condition of 4AD by B. bassiana QY2A was studied.The optimum transformation conditions were obtained: the initial pH value of 6.0, temperature 28℃, 180r/min, the transformation time 60h, the concentration of solution and substrate were 2.5% and 2.5g/L respectively. Then the conversion yield of 11α-OH-4AD was 65%, increased by 51.2%.}