Prokaryotic Expression and Antigenicity Analysis of the Attachment Protein σ1 of Reovirus BYD Strain

Abstract:

The cell attachment protein gene of the S1 segment of reovirus BYD was cloned into prokaryotic expression vector, pET-28a(+). The resulting pET-28a(+)-S1 was transformed into E.coli BL21(DE3) for analyzing whether the constructed vector could express inferred recombinant σ1 and characterizing the antigenicity of the recombinant σ1.SDS-PAGE showed the constructed vector expressed a protein around 53 kilodalton, which was consistent with the deduced molecular weight of the recombinant σ1.And immunoblot assays demonstrated that the recombinant σ1 had good antigenicity and specificity, and therefore it should have great potential in developing diagnosis reagent.