Studies on the Production and Properties of Chitinase from Nomuraea rileyi Strain CQ031021

Abstract:

The producing condition and properties of chitinase secreted by Nomuraea rileyi strain CQ031021 were studied. The optimal conditions for the strain to produce chitinase are 6 days of 28℃ with the initial pH 6.0, and the liquid medium containing 2.0% glucose as its carbon source and 0.6% peptone plus 0.6% beef extract as nitrogen source after inoculating dosage 2mL suspension of conidia (1×10⁷/mL). The optimal temperature and pH for enzyme activity is 50℃ and 6.0, respectively, while the activity can be enhanced by Tween-80 and inhibited by SDS. The enzyme activity is stable under 40℃ and in pH range of 5.5～6.5.