Study on Isolation of Clostridium butyricum from Pits Sludge and Its Characteristics

Abstract:

14 strains were isolated from pits sludge by anaerobic cultivation, two Clostridium butyricum strains were identified by physiological and biochemical experiments and the sequence analysis of 16S rDNA. The physiological characteristics and security of Clostridium butyricum B1 were studied, in vitro research indicated it was tolerant against low pH, bile concentration and antibiotics and has antagonism effects against pathogens.