Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton

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Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton
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A direct lysis manner for extracting DNA directly from rhizosphere soil of transgenic cotton SGK321 and Zhongmiansuo 41 was designed by glass bead-lysozyme-SDS method. The results showed that intact DNA fragments over 20 kb were gained from rhizosphere soil planted two cultivars of transgenic cotton. DNA extracted suited to PCR amplification and RFLP.

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LI Chang-Lin, WU Jian-Bo, YANG Dian-Lin, LIU Wan-Hua. Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton[J]. Microbiology China, 2007, 34(5): 0943-0945

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