Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton

Home > Archive>Volume 34, Issue 5, 2007 >0943-0945

Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton
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                        LI Chang-LinLI Chang-Lin
Agro-environmental Protection Institute, Ministry of Agriculture，Tianjin 300191
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WU Jian-BoWU Jian-Bo
College of Life Science, Nankai University,Tianjin 300071
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YANG Dian-LinYANG Dian-Lin
Agro-environmental Protection Institute, Ministry of Agriculture，Tianjin 300191
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LIU Wan-HuaLIU Wan-Hua
Agro-technique Extension Center，Wuqing District，Tianjin 301700
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Abstract:

A direct lysis manner for extracting DNA directly from rhizosphere soil of transgenic cotton SGK321 and Zhongmiansuo 41 was designed by glass bead-lysozyme-SDS method. The results showed that intact DNA fragments over 20 kb were gained from rhizosphere soil planted two cultivars of transgenic cotton. DNA extracted suited to PCR amplification and RFLP.

Key words:Transgenic cotton, Rhizosphere soil microorganism， RFLP

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LI Chang-Lin, WU Jian-Bo, YANG Dian-Lin, LIU Wan-Hua. Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton[J]. Microbiology China, 2007, 34(5): 0943-0945

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