Characteristics and Molecular Identification of a Strain of Heterotrophic Microorganism Isolated from Acid Mine Drainage

Abstract:

By streak plate method, a strain of heterotrophic microorganism which was extremely acidophilic, alkalitolerant, named by HJM, was isolated from an acid mine drainage of a certain copper mineral in Jiangxi Province. This strain was able to grow under pH value of 1.5～10.0. Morphological characteristics and sequences analysis of 16S rDNA and 26S rDNA D1/D2 region revealed that it belonged to the species P.guilliermondii. Resistant experiment to metals showed that it could resist copper of 45mmol/L, so it was an important strain used to study the resistance mechanism of copper.