Control of Phosphate Concentration on Aminoglycoside Antibiotic JI-20A Fermentation

Abstract:

It was found that the phosphate concentration effected significantly on the cell growth and the production of aminoglycoside antibiotic JI-20A. Although initial phosphate concentration of 6.1mmol/L～9.6 mmol/L was beneficial for the cell growth, the production of aminoglycoside antibiotic JI-20A was inhibited. When the phosphate concentration was controlled below 1.1 mmol/L in the biosynthesis phase of aminoglycoside antibiotic JI-20A, high alkaline phosphatase activity and low pyruvic acid concentration were resulted in, and the production of aminoglycoside antibiotic JI-20A was enhanced.

Key words:Antibiotic JI-20A, Phosphate, Fermentation, Alkaline phosphatase, Pyruvic acid