Novel Mutants of Microbial Glycosidases——Generation and Application of Thioglycoligases

Abstract:

Acid/base mutants of glycosidases, namely thioglycoligases, are able to catalyze thioglycosides synthesis. Now, many thioglycoligases, including β-thioglucoligase, β-thiomannoligase, β-thiogalactoligase, α-thioxyloligase and α-thioglucoligase, have been developed from bacteria and archaebacteria, and applied in synthesizing various thioglycoligases. Recently, thioglycoligases have been used to glycosylate the glycoprotein and firstly generate the thioglycoprotein. The novel extended synthetic function of glycosidases would promote the development of glycobiology, biotechnology and pharmacy.