Study on Detection of Potato Virus A on NC Strips by DAS-ELISA

Abstract:

Based on DAS-ELISA procedures, Dot-ELISA for detecting potato virus A(PVA) was conducted on two kinds of differently processed NC（nitrocellulose） strips (NC strip-1, NC strip-2), respectively. The PVA in infected potato leaves can be clearly detected by performing the ELISA on NC strip-2, whereas not on NC strip-1 due to cross-reaction between two adjacent NC dots. This result has given a deep foundation for developing micro fluidic immunoassay chip for potato virus A.