Cloning and Molecular Characterization of the SLA-2 from Bama Miniature Pig

Abstract:

In order to study the SLA-Ⅰ molecular characterization of the Bama miniature pig which is an eugenic chinese breed, a pair of primers were designed to amplify the SLA-2 (SLA-2*bm) and then the molecular characterization of the interest gene was analyzed by computer. After cloning, sequencing and analyzing by computer, SLA-2*bm was 1119bp and the ORF frame from 3 to 1097 coded 364 amino acids with two sets of disulfide bond in intro-chain constituted by four cysteines situated in 125, 188, 227 and 283 sites. The amino acid identical ratios between SLA-2*bm and other SLA-2, SLA-3 and SLA-1 alleles were 88.4%～96.4%, 88.3%～90.5% and 87.7%～ 92.7%, respectively. The phylogenetic tree of the SLA-2*bm demonstrated that it was relatively independent to other SLA-2 genes and evolved at low extent. Besides, the SLA-2*bm was similar to the HLA-A2 and H-2K in functional domains and it preserved some functional sites of HLA-A2. The results indicated that SLA-2*bm was a new allele of SLA-2 and the Bama miniature pig might keep down the archaic genetic characterization in China.