Structure and Molecular Weight Analysis of Bifidobacterium Peptidoglycan

Abstract:

PG derived from Bifidobacterium thermophilum was separated and purified from the cell wall, and involved in research and analysis of its chemical compositions, structure and molecular weight. The major component of the preparation was validated to be peptidoglycan by animo acid analysis, polysaccharides and protein determination, dissolution by lysozyme. NMR and Infrared spectrum substantiated the glycan chain of peptidoglycan was connected by D-pyranose N-acetylglucosamine and N-acetylmuramic acid through β-1,4 glycosidic bond. SDS-PAGE and multi-stain suggested that the molecular weight of soluble peptidoglycan showed certain diffusibility, and its ranged from lower than 97.6 kD to higher than 14.4 kD.