Optimization of Culture Medium for Laccase Production from Bjerkandera adusta by Uniform Design

Abstract:

Both uniform design and stepwise regression were employed in this study to optimize culture medium for laccase production from Bjerkandera adusta WZFF.W-Y11. The optimal fermentation parameters of B. adusta WZFF.W-Y11 in shaking flask were as follows: starch 24.0g/L, glucose 24.0g/L, bean cake powder 4.8g/L, NH₄Cl 3.2g/L, KH₂PO₄ 3.2g/L, MgSO₄·7H₂O 0.2 g/L, CuSO₄·5H₂O 0.006 g/L, hydrolysate from wheat bran 1％, initial pH6.5, rotating speed 150 r/min, culture temperature 30℃. The activity of laccase reached 9672 U/L.