The Screening of Microorganism Producing Esterase

Abstract:

A strain named ZM1 capable of producing esterase had been isolated from soil samples by the methods of bromocresol purple staining technique and the determination of esterase value. The strain was identified as Absidia glauca Hagem based on its morphological characteristics and the sequence similarity of ribosomal DNA-ITS. A strain named ZZM1 was selected after UV mutagenesis,The results proved that ZMM1 had steady transmissibility,when ZMM1 was cultivated at 32℃ for 48h with 160r/min, the yields of esterase was 58.76U/mL,104.3% higher than ZM1.

Key words:Absidia glauca Hagem,Esterase, Screening, Identification, UV mutagenesis