Primarily Identification of the Strain Bioconverting Monacolin K

Abstract:

In this paper,approximately 1000 strains of actinomycete were screening for their ability for microbial transformation of Monacolin K.Strain ST2710 were capable of bioconverting Monacolin K into hydroxy-Monacolin K.Strain ST2710 was primarily identified using the medium employed by Waksman,Shirling and Gottlieb and the current ISP medium.On Gause's starch agar medium,aerial mycelium form branching filaments and do not show coiling.The utilization of carbohydrates by the ST2710 strain include glucose,sucrose,starch,glycerin etc and determination was made after cultivation at 28℃ for 14 days.It could not decompose milk and liquefy gelatin.It could hydrolysate starch and not grow on cellulose.Strain ST2710 contain major amounts of meso-diaminopimelic acid as the wall diamino acid,arabinose and galactose as major wall sugars;but does not contain mycolic acids.The G＋C content of the DNA is 67.3 mol%.16S rDNA analysis indicated membership of strain ST2710 to the genus Amycolatopsis (99%).Therefore,according to the references,the ST2710 strain was primarily identified as Amycolatopsis sp.