- LIN Fan-Xue
LIN Fan-Xue
(1.The Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070) (2.Department of Biology, Jining Teachers school, Jining 272125)
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Search for this author on this site - CHENG Shui-Ming
CHENG Shui-Ming
Department of Biology, Huanggang Normal University, Huanggang 438000
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Search for this author on this site - LI An-Zheng
LI An-Zheng
The Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070
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Search for this author on this site - LIN Fang-Can
LIN Fang-Can
The Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070
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In this study, 11,150 qualified ESTs(including 10 cDNAs), about 8.1×106 bp in length, were downloaded from ESTs databases of Lentinula edodes in Fungal Genomics Project(FGP) and NCBI. By searching with SSRhunter 1.3 and handwork, 469 SSRs were identified in 316 ESTs, approximately 2.83% of total ESTs. The average distance between SSRs was about 17.3kb. Trinucleotide and hexanucleotide ESR-SSRs were found to be the two most aboundent repeats, accounting for 38.00% and 20.00% of the total ESR-SSRs respectively. The most familiar motifs are(A)n,(T)n,(GA)n,(AG)n,(TGA)n,(GAT)n and(TCTTT)n, which comprised about 35.94% of all EST-SSRs.
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LIN Fan-Xue, CHENG Shui-Ming, LI An-Zheng, LIN Fang-Can. Compose and Distribution of EST-SSR in Genomes of Lentinula edodes[J]. Microbiology China, 2007, 34(3): 0438-0442
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