Isolation, Screening and Identification of Antagonistic Microorganisms Against Magnaporthe grisea

Abstract:

Three hundred and twenty-one bacteria strains were obtained from rice leaves, stem, root tissue and paddy field soil, of which the number of strains which can inhibit mycelium of Magnaporthe grisea growth markedly was fifty-seven through fermentation in 2.0 mL Eppendorf tube, and among these fifty-seven strains, five strains were strongly antagonistic to Magnaporthe grisea. These five strains was identified for their morphologic, physiological and biochemical characteristics, and the results showed that one strain(No.156) was bacillus subtilis, two strains(No.171 and No.177) were Bacillus pumillus and two strains(No.192 and No. 279) were Bacillus ploymyxa.

Key words:Magnaporthe grisea, Antagonistic Microorganisms,Screening,Fermentation,Identification,Bacillus