The Identification of Two Isolates from Cadaver of Brontispa longissima(Gestro) and Their Phylogeny

Abstract:

The strains Dz01 and Ma4 were isolated from cadavers of Brontispa longissima(Gestro), and were confirmed to be pathogenicity to Brontispa longissima(Gestro). After microscopical observation of the morphological characters of mycelium, phialide and conidia from two isolates, they were found to be identical to Metarhizium anisopliae var anisopliae, so they were identificated as M.anisopliae var anisopliae. The Maximum Parsimony tree constructed based on the sequences of ITS1-5.8S-ITS2 regions in ribosomal DNA from two isolates and 31 other isolates which represent different species or varity species of genus Metarhizium obtained from GenBank database showed that two isolates clustered together in the clade which was composed of the isolates classified as Metarhizium anisopliae var anisopliae. This provided the molecular data for the result of morphological identification of Dz01 and Ma4 isolates.