Studies on Simultaneous Detection of Three Gastroenteritis Virus in Clinical Samples by Muptiplex RT-PCR

Abstract:

Rotavirus, norwalk virus and astrovirus are the major causes of viral gastroenteritis. Primers JV12/JV13, P1/P2 and Mon340/Mon348 were used to develop a multiplex RT-PCR system to simultaneously detect norwalk virus, rotavirus and astrovirus in clinical fecal samples. In a total of 128 samples, 62 was rotavirus, 8 was norwalk virus and 11 was astrovirus. The detection limits was 5pg/mL for rotavirus, 50pg/mL for norwalk virus and astrovirus respectively. The multiplex RT-PCR system can be used to detect norwalk virus, rotavirus and astrovirus for routine monitoring and risk assessment in diseases outbreak with high specificity and sensitivity.