The Effect of Dissolved Oxygen on L-Threonine Fermentation

Abstract:

To explore the effects of dissolved oxygen on the fermentation of L-threonine and the method for controlling dissolved oxygen.The influence of dissolved oxygen on the synthesis of L-threonine during fermentation was studied under various volumes，various oxygen supply manners.L-threonine was produced by fed-batch fermentation styles，L-threonine fermentation concentration in fermention broth was determined with the methods of amino acid analysis system.The productivity of L-threonine was 118.9g/L in 10-liter fermentor in 36 h，the conversion rate of glucose is 47.6%.Dissolved oxygen influenced the synthesis of L-threonine significantly.A fermentation process for controlling dissolved oxygen was developed.

Key words:L-threonine，Dissolved oxygen，Fed-batch fermentation