Combination of ARDRA and RAPD Genotyping Technique in Acinetobacter spp.Genomic Species Identification

Abstract:

A total of 10 nonrepetitive multidrug-resistant Acinetobacter strains were collected.With the reference of A.calcoaceticus (ATCC23055)，A.baumannii (ATCC19606) A.lwoffii (ATCC17986) and A.junii (NCTC5866)，DNA fingerprint techniques ARDRA (Amplified ribosomal DNA restriction Analysis) and RAPD (Random amplified polymorphism DNA) were carried out to identify the genomic species of Acinetobacter spp.The distances between them were calculated by unweighted pair group method with arithmetic (UPGMA).Genotypes of Acinetobacter spp.can be effectively classified and an A.junii together with nine A.baumannii isolates was genomically identified.The combination of ARDRA and RAPD DNA-fingerprint technique showed high complementarity，it could be a useful tool in Acinetobacter genomic species identification.