Homologic analysis of 16S rDNA and Elongation Factor Gene of Paulownia Witches'Broom Phytoplasma

Abstract:

A 1.2kb of 16S rDNA length frgement is obtained by nest polymerase chain reaction from paulownia witches phytoplasma samples which were collected from Shaanxi，Shanxi，Gansu，Henan，Hebei，Shandong Province，and also a 850bp DNA fragement of tuf (EF-Tu) gene was amplified from above samples using primers (fTufu/rTufu). Homologic analysis shows that it is the same of 16S rDNA nucleotide sequences of PaWB-Shaanxi，PaWB-Taiwan and that of PaWB from Shanxi，Gansu，Henan，Hebei，Shandong Province.All species of PaWB phytoplasma，from different regions，are believed to be a 16Sr I-D group of phytoplasma.Therefore，we can confirm classified position of PaWB phytoplasma from China.