Studies on the Capacity of Phosphate Dissolving and the Identification of Strain BL-11

Abstract:

The morphology，cultural characteristics，physiological and biochemical properties of phosphate solubilizing strain BL-11 were studied.Combine with its 16S rDNA sequence analysis result strain BL-11 was identified as Brevibacillus laterosporus.Results showed that the phosphate solubilizing efficiency of strain BL-11was up to 10.91% when with Ca₃(PO₃)₂ as the sole phosphorus source in the medium，while the efficiency of that was up to 1.56% when with sands as the sole phosphorus source.An optimal media of BL-11 strain for solubilizing phosphate was obtained by orthogonal test.It was composed of sugar 20g/L，(NH₄)₂HCO₃ 0.3g/L，MgSO₄.7H₂O 0.5g/L，NaCl 0.3g/L，KCl 0.5g/L，FeSO₄ 0.03g/L，MnSO₄.H₂O 0.03g/L. While other conditions were consisted of initial pH7.0-8.0，180 r/min，and 30℃.