Influencing Factors on the Efficiency of Gene Transformation System of the Monascus purpureus 9903A

Home > Archive>Volume 34, Issue 2, 2007 >0279-0282

Influencing Factors on the Efficiency of Gene Transformation System of the Monascus purpureus 9903A
DOI:
                        
CSTR:
                        [cstr]
                    
Author:
                        
Affiliation:
Clc Number:
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

The protoplasts from Monascus purpureus 9903A were transformed with hygromycin B resistance plasmid pMP-Hygro.The transformation medium contained 50 mmol/L Ca²⁺ and 40％PEG4000 with 1.0mol/L sorbitol an osmotic stabilizer.The protoplasts concentration is1×10⁸ /mL and the quantity of vectors is 1 μg/100μL protoplasts.In this study，the average transformation ratio is 160 colonies/μg DNA.

Reference

Cited by

Get Citation

XIA Yong-Jun, NIU Dan-Dan, WANG Zheng-Xiang, XU Gan-Rong. Influencing Factors on the Efficiency of Gene Transformation System of the Monascus purpureus 9903A[J]. Microbiology China, 2007, 34(2): 0279-0282

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online:
Published:

Get Citation

Share

Article Metrics

History

Article QR Code