Isolation and Characterization of a H2-producing Strain Enterbacter sakazakii HP

Abstract:

A H₂-producing bacterial strain was newly isolated and identified as Enterbacte sakazakii HP by 16S rDNA sequence analysis and detection by BBLCRYSTAL AUTOREADER.Various factors，including substrates and its concentration，initial pH，temperature and oxygen，on the hydrogen production of E.sakazakii HP have been studied extensively.Among several sugars，glucose was the favorite substrate for hydrogen production.The optimum condition for hydrogen production by Enterbacte sakazakii HP was achieved as：initial pH8.0，cell density OD₆₀₀=0.7，temperature 35℃，glucose concentration 0.1 mol/L，oxygen concentration 0%.Under batch fermentative hydrogen production conditions，the maximal hydrogen production activity and hydrogen yield were obtained as 5.34μmol H₂/h·mg dw and 1.94mol H₂/mol glucose，respectively.The research results suggest that Enterbacter sakazakii HP is an ideal candidater for biological hydrogen production.